Journal: Current protocols in microbiology

Article Title: Toxoplasma gondii tissue cyst purification using Percoll gradients

doi: 10.1002/cpmc.30

Figure Lengend Snippet: A. Sterilization of mortar and pestle and dissection instruments. B. Mortar and pestle and the dissection instruments including: small curves scissors, small straight scissors, firm rounded tip forceps and microspatula. C. Surface sterilization of euthanized mouse using 70% ethanol on a dissection surface in the biosafety cabinet. Note the 4 liter plastic beaker with bleach in the corner (red asterisk). D. Exposure of the skull by an incision through the skin between the ears. This is followed by the cutting of the skull from the back to the front. E. Lifting the skull cap to the front (skull) exposing the brain. Note that the forceps are used to secure the head holding the skull in the eye sockets. A microspatula is inserted under the skull severing the spinal cord. F. The intact brain is removed using the microspatula.

Article Snippet: list-behavior=simple prefix-word= mark-type=none max-label-size=0 Spray bottle with 70% Ethanol 10× Filter sterilized Phosphate Buffered Saline (PBS) Sterile 1× Phosphate Buffered Saline Sterile 1× PBS with 1% Tween-80 (Sigma P4780) Note: Make 1×PBS by diluting out the 10×PBS and then add Tween-80 to a concentration of 1% 4 Liter Plastic beaker with 2 liters of 10% bleach to be placed in the biosafety cabinet Large low profile ice bucket/pan (Corning/Fisher 432093) Dissection surface (styrofoam lid covered with aluminum foil) Dissection tools (sterilized in an autoclave pouch) ( ) list-behavior=simple prefix-word= mark-type=none max-label-size=0 Sharp fine point scissors –straight and curved Forceps rounded tip Stainless steel microspatula fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 caption a8 Harvesting the brain from infected mice A. Sterilization of mortar and pestle and dissection instruments.

Techniques: Dissection, Hood